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This study investigated the effects of dietary supplementation with Azolla caroliniana and Lemna minor on the growth performance, feed utilization, and survival of juvenile fish. A total of 120 juveniles were randomly assigned to four treatments: T0 (control), T1 (Azolla caroliniana), T2 (Lemna minor), and T3 (combined Azolla caroliniana + Lemna minor). Fish were fed for 60 days, and growth parameters including weight gain (WG), specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), and survival were recorded. Results showed that all supplemented groups exhibited significantly higher growth compared to the control. The combined supplementation group (T3) achieved the highest WG (27.4 ± 1.1 g), SGR (2.41 ± 0.06%/day), and FE (0.70 ± 0.02), while displaying the lowest FCR (1.42 ± 0.04) and highest survival (97.8 ± 1.2%). Correlation and regression analyses confirmed strong positive relationships between aquatic plant biomass and WG, SGR, and FE (r = 0.91–0.96, p < 0.01; R² = 0.94 for Azolla), as well as a negative correlation with FCR (r = ?0.88 to ?0.95), demonstrating enhanced nutrient utilization. Statistical analyses (ANOVA and Tukey’s post hoc test) revealed significant differences among treatments (p < 0.05), and the effect size (?² = 0.68) indicated that a substantial proportion of growth variation was attributable to dietary treatment. The observed improvements in growth performance and feed utilization can be attributed to the high protein content (30–45%) and essential amino acid profile of Azolla caroliniana and Lemna minor, suggesting that these aquatic macrop