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Sustainable aquaculture requires the development of cost-effective and environmentally friendly feed resources to enhance fish growth performance and feed utilization efficiency. Aquatic macrophytes such as Azolla caroliniana and Lemna minor have attracted significant attention due to their high nutritional value, rapid growth rate, and ecological sustainability. This study aimed to statistically evaluate the effects of Azolla caroliniana and Lemna minor biomass supplementation on fish growth performance, including weight gain, length growth, and feed efficiency, under controlled aquaculture conditions. Quantitative statistical analyses were conducted using correlation, regression, ANOVA, and t-test methods to assess the strength, significance, and predictive value of the relationships between aquatic plant biomass and fish growth indicators. Correlation analysis revealed strong positive relationships between total aquatic plant biomass and fish weight gain (r = 0.96), fish length growth (r = 0.93), and feed efficiency (r = 0.91), indicating a high degree of association between plant supplementation and growth performance. Regression analysis further confirmed the predictive capacity of aquatic plant biomass, with coefficients of determination exceeding 0.92, demonstrating that more than 92% of growth variation could be explained by plant biomass availability. ANOVA results showed highly significant effects of biomass supplementation on all growth parameters (F = 42.50, p < 0.001), while t-test analysis confirmed the statistical reliability of these relationships (t = 9.42, p < 0.05). Findings