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PREPARATION AND EVALUATION OF AVIAN INFLUENZA (H9) AND NEWCASTLE DISEASE (THERMOSTABLE I-2 STRAIN) BIVALENT VACCINE FOR COMMERCIAL POULTRY

Shahid Mahmood* and Rabia Sabir
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
*Corresponding author: shahiduaf@gmail.com

The goal of current research was production of bivalent adjuvant based inactivated I-2 strain of Newcastle Disease (ND) and Avian Influenza (AI) vaccine and to compare efficacy with commercially available bivalent inactivated vaccine. Experiment was carried out on one-day-old broiler chicks by dividing them into 3 groups A, B & C. Vaccine was injected on 7, 14, 21 and 28 day. Blood samples were collected on 7, 14, 21, 28 and 35 day. Results of Hemagglutination Inhibition (HI) test presented the geometric mean titers of group A and B were <1:4, 1:18.8, 1:115.2, 1:358.4, 1:128 & <1:4, 1:8.4, 1:36.8, 1:56, 1:22.4 respectively for AI. Similarly the geometric mean titers of group A and B were <1:4, 1:19.6, 1:89.6, 1:320, 1:115.2 & <1:4, 1:9.2, 1:25.6, 1:36.8, 1:22.4, respectively for ND. However, control group showed <1:4 geometric mean titer. The challenge was given on 28th day and birds were monitored for next 7 days for clinical signs. The chicks of group A showed no clinical signs of diseases (AI, ND). However, in group B 60% morbidity and 90% mortality was recorded on 4th and 6th day of post challenge, respectively. While 100% mortality was recorded in control group.

 Keywords: Avian influenza, Bivalent vaccine, Broilers, HI test, Newcastle Disease.

How to cite this article:
Mahmood MS and Sabir R, 2021. Preparation and evaluation of avian influenza (H9) and Newcastle disease (thermostable i-2 strain) bivalent vaccine for commercial poultry. Agrobiological Records 3: 17-23. 
https://doi.org/10.47278/journal.abr/2020.017