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Phytochemicals Analysis and Antimicrobial Activities of Echinops echinatus from Cholistan Desert, Pakistan

Muhammad Rafay1,*, Muhammad Usman Ghaffar1, Muhammad Abid1,*, Zaffar Malik2 and Muhammad Madnee1
1Forestry, Range & Wildlife Management, The Islamia University of Bahawalpur, Pakistan
2Department of Soil Sciences, The Islamia University of Bahawalpur, Pakistan
*Corresponding authors: (MR); (MA)

Plants have been used as a source of food as well as medicines by the man throughout history. Echinops echinatus was evaluated for phytochemicals and antibacterial activities against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Anti-urease inhibition assay activities were tested during the study. The laminar flow cabin was cleaned with 70% ethanol. α-glucosidase inhibition activity was performed according to the standard method with slightly modification. Carbohydrates, phenols and tannins were found positive in leaves, stems and roots. Saponins and glycosides were found in flowers, leaves, roots and stems. While roots and stems revealed the presence of steroids. Terpenoids were found in flowers and leaves. The leaves showed the maximum inhibition zone 10mm against K. pneumonia while against E. coli, S. aureus and P. aeruginosa was found inactive. K. pneumoniae, E. coli and P. aeruginosa were resistant to flowers extract. The root extract showed maximum zone of inhibition 18 mm against S. aureus while against P. aeruginosa and K. pneumonia inhibition zone was 15mm and 14mm respectively. The extracts of leaves, flowers, roots and stem showed positive results for α-glucosidase. The roots extract showed maximum inhibition with 75.3±1.5 with IC50 value of 207.3±1.3 for α-glucosidase. Urease inhibitory activity of stem extracts showed maximum inhibition activity 93.71±0.86 with IC50 value 15.63±0.42. While leaves and roots showed almost same activity 92.45±0.63 and 92.63±0.76, respectively. Echinops echinatus could be considered for further studies in the treatment of various ailments as a natural remedy.

 Keywords: Echinops echinatus, Klebsiella, Pseudomonas, Staphylococcus, E. coli, Anti-urease, Anti-glucosidase

How to cite this article: 
Rafay M, Ghaffar MU, Abid M, Malik Z and Madnee M, 2021. Phytochemicals Analysis and Antimicrobial Activities of Echinops echinatus from Cholistan Desert, Pakistan. Agrobiological Records 5: 21-27.