Abstract

HEMATOLOGICAL, SERUM BIOCHEMISTRY, HISTOPATHOLOGICAL AND MUTAGENIC IMPACTS OF TRICLOSAN ON FISH (BIGHEAD CARP)

Rabia Akram1, Abdul Ghaffar2, Riaz Hussain3,*, Iahtasham Khan4, Vania Lucia de Assis Santana5, Khalid Mehmood6, Saima Naz7, Rehana 1qbal1, Hafiz Muhammad Imran3, Muhammad Rafi Qamar3 and Hongyun Zhu8

 1Institute of Pure and Applied Biology, Zoology Division, Bhauddin Zakariya University, Multan, Pakistan; 2Department of Life Sciences (Zoology), The Islamia University of Bahawalpur- 63100, Pakistan; 3Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur- 63000, Pakistan; 4Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore Sub-Campus, Jhang, Pakistan; 5National Agricultural Laboratory of Pernambuco, Ministry of Agriculture, Livestock and Food Supply of Brazil. Rua Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco CEP 52171-030, Brazil; 6Department of Clinical Medicine and Surgery, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur- 63000, Pakistan; 7Department of Zoology, Government Sadiq College Women University, Bahawalpur Pakistan; 8Key Laboratory of Clinical Veterinary Medicine in Tibet, Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, People’s Republic of China.

*Corresponding Author: dr.riaz.hussain@iub.edu.pk

Agro-aquatic ecosystems are mainly and persistently exposed to a variety of unwanted chemicals and pollutants due to indiscriminate use of agrochemicals such as pesticides, insecticides, heavy metals, germicides, drug residues, industrial wastes, and feed additives. In the present experimental trial, 16 active fish (bighead carp) were randomly kept in four unlike groups (A to D). Blood and serum were obtained on days 5, 10, and 15 of the experiment. For histopathological alterations, fish in every individual group were slaughtered on day 15 of the trial. Mild to moderate different physical ailments like jerking movement, erratic swimming, and mucous secretion from the mouth of fish kept in group D were observed after day 10 of post-exposure. Significantly, lower values of erythrocyte count, Hb, hematocrit while increased values of total white blood cells and neutrophil counts were recorded in fish of groups (C-D). Results on serum chemistry showed a significantly increased quantity of liver functional tests (ALT and AST), renal functional tests (urea and creatinine), and cardiac biomarkers. Results on micronuclei and comet assay indicated increased frequency of DNA damage. The frequency of nuclear and morphological variations in RBCs of fish of group (D) significantly increased compared to the control group. Results on microscopic levels exhibited different histopathological alterations in gills (twisting of secondary lamellae, uplifting of lamellae, lamellar disorganization, necrosis of lamellar epithelial cells), liver (congestion, necrosis of hepatocytes, fatty infiltration), brain (necrosis and atrophy of neuron) and kidneys (necrosis of tubules, increased urinary spaces, tubular necrosis) in treated fish in groups (C-D) after day 10 of post-exposure. From the findings of our experimental research, it can be suggested that triclosan causes toxic effects in bighead carp.

Keywords: Bighead Carp, triclosan, blood, Serum, DNA damage, histopathology

How to cite this article: 
Akram R, Ghaffar A, Hussain R, Khan I, de Assis Santana VL, Mehmood K, Naz S, Iqbal R, Imran HM, Qamar MR and Zhu H, 2021. Hematological, serum biochemistry, histopathological and mutagenic impacts of triclosan on fish (Bighead carp). Agrobiological Records. https://doi.org/10.47278/journal.abr/2021.009